SPEAKERS
CV (list 1) .................. see
list 2
Prof.
Juan J. CALVETE
Full
Professor; Head of the Structural Proteomics Laboratory. Instituto
de Biomedicina de Valencia, C.S.I.C., Jaime Roig 11,
46010-Valencia (Spain). Phone: +34 96 339 1778; Fax: +34 96 369
0800; Email: jcalvete@ibv.csic.es
Lecture
Title: 'Snake
venomics and antivenomics: from ophidian evolution to biomedicine'
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1998-
Staff Researcher at Researcher at Instituto de Biomedicina,
CSIC (Spain) (Structural Proteomics)
2001- Professor at Universidad de la República (Uruguay)
(Biology)
1993-98:
Staff Researcher at Veterinary Highschool, Hannover (Ger) (Structural
Biology)
1998- Staff Researcher at Researcher at Instituto de Biomedicina,
CSIC (Spain) (Structural Proteomics)
2001- Professor at Universidad de la República (Uruguay)
(Biology)
PROFESSIONAL
POSITIONS
Since 1989 Established Investigator of the Spanish Research Council
(CSIC)
1993-1998 Staff Researcher of the Veterinary High School of Hannover,
Germany
AWARDS
1989 Young Scientist Award of the International Society on Thrombosis
and Haemostasis
PUBLICATIONS
(Selected publications from the last 5 years, out of 260 papers
in peer-reviewed journals and book chapters since 1986)
Obtustatin, a potent and selective inhibitor of 1 1 integrin in
vitro and angiogenesis in vivo
Marcinkiewicz, C., Weinreb, P.H., Calvete, J.J., Kisiel, D.G.,
Mousa, S.A., Tuszynski, G.P. & Lobb, R.R.Cancer Res. 63 (2003)
2020-2023
NMR
solution structure of the non-RGD disintegrin obtustatin. Moreno-Murciano,
M.P., Monleón, D., Marcinkiewicz, C., Calvete, J.J. &
Celda, B. J. Mol. Biol. 329 (2003) 135-145
Snake
venom disintegrins. Novel dimeric disintegrins and structural
diversification by disulphide bond engineering. Calvete, J.J.,
Moreno-Murciano, M.P., Theakston, R.D.G., Kisiel, D.G. & Marcinkiewicz,
C. Biochem. J. 372 (2003) 725-734
Concerted
motions of the integrin-binding loop and the C-terminal tail of
the non-RGD disintegrin obtustatin. Monleón, D., Moreno-Murciano,
M.P., Kovacs, H., Marcinkiewicz, C., Calvete, J.J. & Celda,
B. J. Biol. Chem. 278 (2003) 45570-45576
Snake
venomics: characterization of protein families in Sistrurus barbouri
venom by cysteine mapping, N-terminal sequencing, and MS/MS analysis.
Juárez, P., Sanz,L. & Calvete, J.J. Proteomics 4 (2004)
327-338
Structural
requirements of MLD-containing disintegrins for distinct inhibition
of 4 and 9 integrins
Bazan-Socha, S., Kisiel, D.G., Young, B., Theakston, R.D.G., Calvete,
J.J., Sheppard, D. & Marcinkiewicz, C. Biochemistry 43 (2004)
1639-1647
Conformation
and concerted dynamics of the integrin binding site and the C-terminal
region of echistatin revealed by homonuclear NMR. Monleón,
D., Esteve, V., Kovacs, H., Calvete, J.J. & Celda, B. Biochem.
J. 387 (2005) 57-66
Structure-function
correlations of snake venom disintegrins. Calvete, J.J. Curr.
Pharm. Design 11 (2005) 829-835
Snake
venomics: comparative analysis of the venom proteomes of the Tunisian
snakes Cerastes cerastes, Cerastes vipera and Macrovipera lebetina.
Bazaa, A., Marrakchi, N., El Ayeb, M., Sanz, L. & Calvete,
J.J.Proteomics 5 (2005) 4223-4235
The
first crystal structure of a Mimosoideae lectin reveals a novel
quaternary arrangement of a widespread domain. Gallego del Sol,
F., Nagano, C.S., Cavada, B.S. & Calvete, J.J.
J. Mol. Biol. 353 (2005) 574-583
cDNA
cloning and functional expression of jerdostatin, a novel RTS-disintegrin
from Trimeresurus jerdonii and a specific inhibitor of the 1 1
integrin
Sanz, L., Chen, R-Q., Pérez, A., Hilario, R., Juárez,
P., Marcinkiewicz, C., Monleón, D., Celda, B., Xiong, Y-L.,
Pérez-Payá, E. & Calvete, J.J., J. Biol. Chem.
280 (2005) 40714-40722
Conformational
diversity of the Goodpasture antigen, the noncollagenous-1 domain
of the 3 chain of collagen IV. Calvete, J.J., Revert, F., Blanco,
M., Cervera, J., Tárrega, C., Sanz, L., Revert-Ros, F.,
Granero, F., Pérez-Payá, E., Hudson, B.G. &
Saus, J.
Proteomics 6 (2006) S237-S244 (Proteomics 8 (2007) 217)
Molecular
cloning of disintegrins from Cerastes vipera and Macrovipera lebetina
transmediterranea venom gland cDNA libraries. Insight into the
evolution of the snake venom´s integrin inhibition system.
Sanz, L., Bazaa, A., Marrakchi, N., Pérez, A., Chenik,
M., Bel Lasfer, Z., El Ayeb, M. & Calvete, J.J., Biochem.
J. 395 (2006) 385-392
Characterizing
the tick carboxypeptidase inhibitor: Molecular basis for its two-domain
nature
Arolas, J.L., Bronsoms, S., Ventura, S., Avilés, F.X. &
Calvete, J.J., J. Biol. Chem. 281 (2006) 22906-22916
Venom
proteomes of closely-related Sistrurus rattlesnakes with divergent
diets
Sanz, L., Gibbs, H.L., Mackessy, S.P. & Calvete, J.J.J. Proteome
Res. 5 (2006) 2098-2112
Snake
venomics of Bitis gabonica gabonica. Protein family composition,
subunit organization of venom toxins, and characterization of
dimeric disintegrins bitisgabonin-1 and bitisgabonin-2
Calvete, J.J., Marcinkiewicz, C. & Sanz, L.
J. Proteome Res. 6 (2007) 326-336
KTS-
and RTS-disintegrins: anti-angiogenic viper venom peptides specifically
targeting the 1 1 integrin Calvete, J.J., Marcinkiewicz, C. &
Sanz, L. Curr. Pharm. Des. 13 (2007) 2853-2859
Snake
venomics. Protocols and applications. Calvete, J.J., Juárez,
P. & Sanz, L.J. Mass Spectrom. 42 (2007) 1405-1414
Snake
venomics of Bitis species reveals large intragenus venom toxin
composition variation. Application to taxonomy of congeneric taxa.
Calvete, J.J., Escolano, J. & Sanz, L.
J. Proteome Res. 6 (2007) 2732-2745
Insights
into the structural basis of the pH-dependent dimer-tetramer equilibrium
through crystallographic analysis of recombinant Diocleinae lectins.
Nagano, C.S., Calvete, J.J., Barettino, D., Pérez, A.,
Cavada, B.S. & Sanz, L.
Biochem. J. 409 (2008) 417-428
Snake
venomics of Central American pitvipers. Clues for rationalizing
the distinct envenomation profiles of Atropoides nummifer and
Atropoides picadoi. Angulo, Y., Escolano, J., Lomonte, B., Gutiérrez,
J.M., Sanz, L. & Calvete, J.J. J. Proteome Res. 7 (2008) 708-719
Snake
venomics of the South and Central American Bushmasters. Comparison
of the toxin composition of Lachesis muta gathered from proteomic
versus transcriptomic analysis
Sanz, L., Escolano, J., Ferretti, M., Biscoglio, M.J., Rivera,
E., Crescenti, E.J., Angulo, Y., Lomonte, B., Gutiérrez,
J.M. & Calvete, J.J. J. Proteomics 71 (2008) 46-60
CURRENT RESEARCH INTEREST
Structural Biology: Carbohydrate-binding proteins -spermadhesins
(sperm surface-coating lectins involved in sperm physiology) and
plant lectins; Non-conventional Proteomics; Snake venomics and
antivenomics
Memberships
in Professional Societies
Spanish Biophysical Society (1996-); Protein Society (1997-);
American Association for Cell Biology (1997-); Biochemical Society
(1999-); Spanish Society for Biochemistry and Molecular Biology
(1999-); Spanish Proteomics Society (2004-); Spanish Society of
Mass Spectrometry (2005-); International Society of Toxinology
(2005-); American Society for Biochemistry and Molecular Biology
(2006- ); Asociacion Española de Reproduccion Animal (2006-
); President of the Spanish Proteomics Society (http//:www.cbm.uam.es/seprot)
REVIEWER OF MANUSCRIPTS FOR:
Journal of Cell Biology, European Journal of Biochemistry/FEBS
Journal, Biochemical Journal, Biological Chemistry Hoppe-Seyler,
Andrologia, Journal of Experimental Animal Science, Reproduction
in Domestic Animals, Toxicon, Fertilität, Thrombosis Research,
Thrombosis and Haemostasis, FEBS Letters, Journal of Chromatography,
Canadian Jounal of Physiology and Pharmacology , Electrophoresis,
Fecundation, Acta Crystallographica Section D, Biotechnology and
Applied Biochemistry, Journal of Biological Chemistry, Biochimica
et Biophysica Acta, Archives of Biochemistry and Biophysics, Biophysical
Journal, Structure, Proteomics, Protein Science, Journal of Physical
Chemistry, Current Pharmaceutical Design, Biochemistry , Plant
Molecular Biology, Journal of Thrombosis and Haemostasis, International
Journal of Biological Macromolecules, Gene, Glycobiology, Genetics,
Journal of Proteome Research, Journal of Pharmacy and Pharmacology,
Comparative Biochemistry and Physiology, Cellular and Molecular
Life Sciences, IUBMB Life, PROTEINS: Structure, Function &
Bioinformatics, Molecular Reproduction and Development, Molecular
Human Reproduction, Experimental Biology and Medicine, Planta
Medica, Molecular and Cellular Proteomics, Journal of Mass Spectrometry,
Journal of Experimental Biology, PROTEÓMICA-Revista de
la Sociedad Española de Proteómica, Protein and
Peptide Letters, Journal of Molecular Evolution, Carbohydrate
Research, Biomacromolecules, PLoS ONE Medicine, BMC Genomics,
Journal of Proteomics, Molecular Biology Reports
REVIEWER
OF GRANT APPLICATIONS FOR:
Agencia Nacional de Evaluacion y Prospección, Ministerio
de Ciencia y Tecnología, Programa de Proyectos Intramurales
de Frontera 2006 del Consejo Superior de Investigaciones Científicas,
Instituto de Salud Carlos III, Generalitat Valenciana, Fundación
Séneca de la Agencia de Ciencia y Tecnología de
la Región de Murcia, Organisation du Traite de L'Atlantique
Nord (OTAN), International Foundation for Science (IFS, Stockholm),
European Science Foundation (Strasbourg), Israel Science Foundation,
Programa de Desarrollo Tecnológico del Ministerio de Educación
y Cultura (Montevideo, Uruguay); International Polar Year o Netherlands
Programme (IPYoNL) Netherlands Organisation for Scientific Research
(NWO); Human Frontier Science Program Organization
MEMBER OF EDITORIAL BOARDS:
EDITORIAL ADVISER of BIOCHEMICAL JOURNAL (1999-)
EDITORIAL BOARD MEMBER of TOXICON (2006- )
EDITOR-IN-CHIEF OF JOURNAL OF PROTEOMICS (2007- )
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Prof.Chidi
Gideon Osuagwu
Department
of Biomedical Technology
School of Health Technology, Federal University of Technology.
P.M.B. 1526, Owerri, Imo state, Nigeria
E-mail: chidi.osuagwu@gmail.com
Lecture
Title: 'Oxidative
stress; sicklecell syndrome as model'
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EDUCATION:
1. PhD (Biochemistry, 1993) University of Port-Harcourt, Nigeria.
Dissertation on "Blood Redox Changes in Sickle Cell Disease"
2. Post-graduate studies in Medicinal Chemistry, University of Houston,
Texas (1978-79).
3. MSc (Chemistry, 1978) Youngstown State University, Ohio.
4. BSc (Biology/Chemistry, 1975) Indiana University of Pennsylvania,
Indiana Pennsylvania (Norah E Zink University Scholar, 1972-1975).
MEMBERSHIP OF PROFESSIONAL ASSOCIATIONS:
1. Fellow, Institute of Chartered Chemists of Nigeria (FICCON)
2. Member, Science Teachers Association of Nigeria (STAN)
3. Member, Nigerian Society of Biochemistry and Molecular Biology
(NSBMB).
4. Member, Chemical Society of Nigeria (C.S.N).
5. Associate Member, Nigerian Institute for Biomedical Engineering
(NIBES).
AREAS OF CURRENT RESEARCH INTEREST.
1. Investigation into the Bioenergetics/Redox aspects of Diseases;
Sicklecell, Diabetes, Redox stress, Malaria, HIV?AIDS, etc.
2. Investigation into the relationship of structure and function
in blood and other biological systems.
3. Studies on the chemistry of African traditional herbs/foods.
4. Investigation into the kinetic molecular origin of natural constants
(Epsilon, Pi and Feigenbaum's number)
5. Studies on African Cosmology/indigenous Knowledge system in relation
to Science.
PUBLICATIONS.
Have published sizable journal articles/books in Chemistry/Biochemistry/Systems'
modeling, and African Cosmology.
SPECIAL RECOGNITION
1. Listed by Philosopedia (see Chidi Gideon Osuagwu at www.philosopedia.org).
2. Nomination to UNESCO in 2005 by the Imo State Government as a
"Living Human Treasure" for work in African Cosmology
(see www.unescong.org/Living Human Treasures).
JOB EXPERIENCE:
1. Currently, Senior Lecturer (Biochemistry), School of Health Technology,
Federal University of Technology, Owerri, Nigeria. Have taught,
since 1978, in different higher institutions in the United States
and Nigeria.
CURRENT CIVIC/ADMINISTRATIVE RESPONSIBILITIES:
1. Member, Board of Directors, Alvana Community Bank (2001-date)
2. Member, Scientific Council, Whelan Research Academy, Owerri,
Nigeria.
3. Head, Department of Biomedical Technology, Federal University
of Technology, Owerri, Nigeria. |
Dr.
EL MOUALIJ Benaïssa
Scientific
Director-Lecturer
Laboratory of Human Histology-CRPP, Institute of Pharmacy-CHU,
Avenue de l'Hôpital, 1, Sart Tilman 4000, Liège,
Belgium.Tel:00-32-4-366-43-28, Fax:00-32-4-366-43-21. E-mail:
b.elmoualij@ulg.ac.be,
Alternative:elmoualijb@hotmail.com
Sit web: http://www.ulg.ac.be/histohum
Lecture
Title: 'Prion:
a splendid and fatal molecular machine'
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Current
position: Since 2002 Research Scientist and Scientific Director
at the Department of Human Histology- Centre of Research on Prion
Protein "CRPP". University of Liège, Belgium.
EDUCATIONAL
BACKGROUND:
1994-1999
Ph.D. in Biochemistry, University of Liège, Belgium.
1992-1994 Master. Sc. in Biochemistry, University of Liège,
Belgium.
1987-1992 B. Sc. in Biochemistry, University Moulay Ismail of
Méknès, Morocco.
EMPLOYMENT
HISTORY:
2002-
Present: Scientifc Director, Centre of Research on Prions Proteins,
University of Liège
2000-2002 Research Scientist, CRPP, University of Liège
1999-2000 Postdoctoral affiliate, Center of protein engineering,
University of Liège
1994-1999 Phd assistant-student, Laboratory of Bioenergetics,
University of Liège.
PUBLICATIONS IN RELATION WITH THE CONFERENCES:
1
Ingrid Dupiereux, Willy Zorzi, Olivier Thellin, Olivier Pierard,
Nicole Watt, NM Hooper, Ernst Heinen and Benaïssa ELMoualij.
Potent protective effect of prion proteins on paraquat-induced
mitochondrial uncoupling in Human Neuroblstoma SH-SY5Y cells.
Journal of neurosciences research, 2008 86:653-659.
2
Dupiereux I, Zorzi W, Danièle Zorzi, Rachidi Walid, Bernard
Lheureux, Heinen E, Elmoualij B. Study on the toxic mechanism
of prion protein peptide 106-126 in neuronal and non neuronal
cells J Neurosci Res. 2006 Aug 15;84(3):637-46.
3
Falisse-Poirrier N, Ruelle V, Elmoualij B, Zorzi D, Pierard O,
Heinen E, De Pauw E, Zorzi W. Advances in immunoproteomics for
serological characterization of microbial antigens. J Microbiol
Methods. 2006 Dec;67(3):593-6. Epub 2006 Jul 5.
4
Dupiereux I, Zorzi W, Lins L, Brasseur R, Colson P, Heinen E,
ElMoualij B.
Interaction of the 106-126 prion peptide with lipid membranes
and potential implication for neurotoxicity. Biochem Biophys Res
Commun. 2005 Jun 17; 331(4):894-901.
5
Stéphanie GOFFLOT, Manuel DEPREZ, Benaïssa EL MOUALIJ,
Awad OSMAN, Jean-François THONNART, Olivier HOUGRAND, Ernst
HEINEN, Willy ZORZI. Immuno-quantitative PCR for Prion Protein
detection in Sporadic Creutzfeldt-Jakob Disease. Clinical chemistry.
2005 Sep;51(9):1605-1611.
6
Gofflot S, El Moualij B, Zorzi D, Melen L, Roels S, Quatpers D,
Grassi J, Vanopdenbosch E, Heinen E, Zorzi W. Immuno-quantitative
polymerase chain reaction for detection and quantitation of prion
protein. J Immunoassay Immunochem. 2004;25(3):241-58.
7-
Jean Barbeau, Benaïssa El Moualij, Ernst Heinen, Willy Zorzi
. TRANSMISSION ET RÉSISTANCE DES PRIONS : LA PRATIQUE DE
LA MÉDECINE DENTAIRE EN SERA T-ELLEAFFECTÉE ? Journal
de l'Ordre des dentistes du Québec Volume 43 Décembre
2006, 43(9) : 461-467.
PATENTS:
1-
ElMoualij Benaïssa, Zorzi Willy, Zorzi Danièle, Heinen
Ernst, Melen Laurence, Detection method by PCR, WO0131056, 2001-05-03,
IqPCR using a chimeric DNA marker for amplification, WO0131056,
2001-05-03 ; EP1232283, 2002-08-21 ; DE60028233T, 2007-03-08.
2- ElMoualij Benaïssa, Zorzi Willy, Fabienne Eppaillard,
Gilbert Legeay, Heinen Ernst. New supports for immunodetection
and the storage or to the selective capture of molecules of interest,
especially of prion proteins. WO2006105622, 2006-10-12
LIST
OF MAIN RESEARCH GRANTS:
1-
Development of an highly sensitive immuno-detection methods for
prions proteins. 2000-2004.
2- Monitoring and elimination of the prions in the effluents and
the environment of the slaughter-houses. 2003-2006.
3- Study of proteinopathies: from oxidative stress to therapeutics.
2004-2006.
4- Development of a "spin off, I-Detect" dedicated to
proteinopathies. 2004-2006.
5- Neuroscreen: Developpement of a differential markers of Alzheimer,
Parkinson and prions diseases. STREP project. 2006-2009.
MEMBER
OF SCIENTIFIC SOCIETIES
Belgian
Society of Biochemistry and Molecular Biology (SBB).
French society of biomaterials (BIOMAT).
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Prof.
Miguel A. De La Rosa
President-elect
of the Spanish Society of Biochemistry and Molecular Biology (SEBBM)
and Full Professor of Biochemistry and Molecular Biology at the
University of Seville.
Instituto
de Bioquímica Vegetal y Fotosíntesis, Universidad
de Sevilla y CSIC, Avda. Américo Vespucio, 49 41092-Sevilla,
Spain
Tel: 34 954 489510, Fax: 34 954 460065. Email: marosa@us.es
Lecture
Title: 'Protein
Evolution and Geochemical Changes'
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He
is the founder and head of the Laboratory of Structural and Functional
Proteomics, Institute of Plant Biochemistry and Photosynthesis,
University of Seville and National Research Council (CSIC), Spain.
His
major research interests are in the structure-function relationships
of metalloproteins, protein evolution, photosynthesis and bioenergetics.
He is author or co-author of 4 books, 2 patents, and over 140
original articles and book chapters, and has been invited speaker
in more than 100 scientific meetings. He is/has been member of
the FEBS Advanced Courses Committee (2003-06) and of the IUBMB
Nominating Committee (2003-06), former President (2003-07) and
Secretary General (1996-2001) of the Bioelectrochemical Society,
editor of FEBS Letters (2005-), associate editor of Bioelectrochemistry
(2005-), President of the 22nd IUBMB & 37th FEBS Congress
(Seville, 2012).
Five
Selected Publications (in the last few years):
Photosynthesis:
A New Function for an Old Cytochrome?
F.P. Molina-Heredia, J. Wastl, J.A. Navarro, D. Bendall, M. Hervás,
C. Howe & M.A. De la Rosa.
Nature (2003) 424, 33-34.
Electron
Transfer between Membrane Complexes and Soluble Proteins in Photosynthesis
(invited review).
M. Hervás, J.A. Navarro & M.A. De la Rosa.
Accounts of Chemical Research (2003) 36, 798-805.
Structure
of the Complex between Plastocyanin and Cytochrome f from the
Cyanobacterium Nostoc Sp. PCC 7119 as Determined by Paramagnetic
NMR.
I. Díaz-Moreno, A. Díaz-Quintana, M.A. De la Rosa
& M. Ubbink.
The Journal of Biological Chemistry (2005) 280, 18908-18915.
Thermal
Unfolding of Plastocyanin from the Mesophilic Cyanobacterium Synechocystis
sp. PCC 6803 and Comparison with its Thermophilic Counterpart
from Phormidium laminosum.
M. Feio, A. Díaz-Quintana, J.A. Navarro & M.A. De la
Rosa.
Biochemistry (2006) 45, 4900-4906.
A
Proteomic Approach to Iron and Copper Homeostasis in Cyanobacteria
(invited review).
B. De la Cerda, O. Catielli,R.V. Durán, J.A. Navaro, M.
Hervás & M.A. De la Rosa.
Briefings in Functional Genomics and Proteomics (2008)6, 322-329.
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Prof.
Vincent Pryde Kehdingha TITANJI
Biotechnology
Unit, University of Buea, Box 63, Buea, SW Province, Cameroon.
E-mail: vpk.titanji@yahoo.com
Lecture
title: 'Molecular cloning and characterization of UB 05 a
dominant antigen of Plasmodium falciparum with immune protective
potential'
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Prof.
Vincent P.K. Titanji obtained his PhD degree (1978) and 'Docent'
title (1988) in Physiological Chemistry from Uppsala University
and his MSc degree in Animal Biochemistry from Lomonosov Moscow
State University (1973). Subsequently, he founded and headed the
Biotechnology Center, Nkolbisson, University of Yaounde I (1986-1992).
He was appointed to the University of Buea as Dean of the Faculty
of Science (1993-1998) Deputy Vice-Chancellor for Teaching(1998-2005)
and Vice Chancellor since 2006.
An
expert in Protein Chemistry and Molecular Parasitology, he has
devised diagnostic tests for river blindness, cloned vaccine candidates
and identified new drug leads from medicinal plants for river
blindness and malaria. He successfully trained 18 PhD graduates,
60 MSc students and has published widely in peer-reviewed journals.
Prof.
Titanji was instrumental in the creation of the Cameroon Biochemical
Society and has been President and Secretary General respectively
of the Federation of African Societies of Biochemistry and Molecular
Biology (FASBMB) and the Federation of African Immunological Societies
(FAIS). A Knight of the Cameroon Order of Valour, Prof. Titanji
is a Chartered Biologist (C. Biol), and Fellow (FI Biol) of the
Institute of Biology, London; a Fellow of the Third World Academy
of Sciences (FTWAS) and a recipient of the IFS/DANIDA Prize.
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Matilde
Barón
Department
of Biochemistry, Cell and Molecular Biology of Plants. Estación
Experimental del Zaidín. Profesor Albareda, 1. 18008 Granada,
Spain
E-mail:
matilde.baron@eez.csic.es
Lecture
Title:"Proteomic
and imaging analysis of pathogen-challenged plants"
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Current
position: Since 2006 Research Scientist at the Department of Biochemistry
and Molecular Biology of Plants, Estación Experimental
del Zaidín (EEZ), Spanish Council of Scientific Research
(CSIC), Granada, Spain.
Graduated
in Biological Sciences, with Special Award from the UGR. University
of Granada (UGR, Spain) in 1978.
PhD in in Biological Sciences, with Special Award from the UGR.
University of Granada. Spain 1984
Postdoctoral researcher at the Dept. of Plant Physiology and Plant
Biochemistry University of Konstanz. Germany. 1985-1987.
Scientific Researcher. Department of Molecular Biology, Biochemistry
and Cell Biology of Plants. EEZ. CSIC. 1988- 2006.
Research
Leader
of the group Response of the chloroplast against biotic and abiotic
stress.
Key words: photosynthesis, plant stress, plant pathogens, heavy
metal toxicity, imaging techniques, chloroplast proteomics.
The group has a very dynamic national and international cooperation
with countries as Morocco, Germany, Czech Republic, Hungary, Bulgaria,
France. The group leader has obtained some international cooperation
awards, as the Gabor Farkas one, from the Hungarian Scientia Amabilis
Foundation.
Selected
publications
M.
L. Pérez-Bueno, J. Rahoutei, C. Sajnani, I. García-
Luque and M. Barón.
Proteomic analysis of the oxygen-evolving-complex of Photosystem
II under biotic stress. Studies on Nicotiana benthamiana infected
with tobamoviruses. Proteomics, 4: 418-25, 2004.
Abdelmalek
Hakmaoui, Matilde Barón, Mohammed Ater.
Environmental biotechnology. Screening Cu and Cd tolerance in
Salix species from North Morocco. African. Journal of Biotechnology
5 (13), pp.1299-1302, 2006.
L.
Chaerle, M. Pineda, D. Van Der Straeten, M. Barón
Robotized thermal and chlorophyll-fluorescence imaging of pepper
mild mottle virus infection in Nicotiana benthamiana. Plant Cell
Physiology. 47, 1323-1336, 2006.
M.
L. Pérez-Bueno, M. Ciscato, M. vandeVen, I. García-Luque,
R. Valcke, M. Barón. Imaging viral infection. Studies on
Nicotiana benthamiana plants infected with the pepper mild mottle
tobamovirus Photosynthesis Research, 90: 111-123, 2006.
Carlota
Sajnani, Jorge L. Zurita, Mercedes Roncel, José M. Ortega,
Matilde Barón, Jean-Marc Ducruet. Changes in photosynthetic
metabolism induced by tobamovirus infection in Nicotiana benthamiana
studied in vivo by chlorophyll thermoluminescence. New Phytologist
175:120-130, 2007.
Rodríguez-Moreno
L., Pineda M., Soukupová J, Macho, A, Beuzón C.R.,
Barón M , Ramos C. Early detection of bean infection by
pseudomonas syringae in asymptomatic leaf areas using chlorophyll
fluorescence imaging. Phot. Res, 96:27-35, 2007. (
Mónica
Pineda, László Gáspár, Fermín
Morales, Zoltán Szigeti, Matilde Barón. Multi-colour
fluorescence imaging: an useful tool to visualise compatible viral
infection in plants (Photochemical Photobiology, 2008).
Selected
projects as project leader
Supported
from the Spanish Research Agencies
-Analisys of plant defence mechanisms against virus infection
and supression of photosynthesis damage (BIO98-0860-CO2-02), CICYT,
National Programme of Biotecnology. 1999/2001.
-Viral infections as biotic stress factor in photosynthesis. Metabolic
changes and defense mechanism studies on infected leaves (BIO2001-1937-C02-02).
MCYT, National Biotechnology Programme (Spain), 2001/2004.
-The chloroplast as target of the viral infection (BIO2004-04968-C02-02).
MCyT. National Biotechnology Programme (Spain), 2004/2007.
- Evaluation of the impact of pathogen infection on the host plant
by imaging and proteomic techniques . MEC (BIO2007-67874-C02-02),
2007-2008.
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Dominique
ROBY
Directeur
de Recherches CNRS. Leader of the group " Resistance, susceptibility
and cell death in Arabidopsis thaliana in response to pathogenic
bacteria "
Institute : Laboratoire des Interactions Plantes/Microorganismes
(LIPM), UMR INRA/CNRS 2594, Chemin de Borde Rouge, BP 52627, 31326
Castanet-Tolosan Cedex, France (http://www2.toulouse.inra.fr/centre/lipm).
Tél. : 05 61 28 53 26, E-mail : dominique.roby@toulouse.inra.fr
Lecture
Title: '
Key genes involved in resistance and hypersensitive cell death
in Arabidopsis'
|
|
Director
of the Federative Institute of Research " Agrobiosciences,
Interactions and Biodiversity " of Toulouse
(http://www2.toulouse.inra.fr/ifr40/fr/1_Presentation/index.htm)
Education
and Career History
Master
of Plant Physiology, University Paul Sabatier, Toulouse, France,
1978.
PhD thesis in Plant Pathology, Université Paul Sabatier,
France, 1981.
Doctorat d'Etat, Université Paul Sabatier, Toulouse, France,
1987.
Postdoctoral Research Associate (1987-1988), Department of Biological
Sciences, Rutgers University, Newark, New-Jersey, USA
Postdoctoral Research Associate (1988-1989) E. DuPont de Nemours,
Department of Agricultural Products, Experimental Station, Wilmington,
Delaware, USA
Scientific Researcher CNRS Laboratoire de Physiologie Végétale,
Toulouse, France (1982-1986) and Laboratory of Plant-Microorganism
Interactions (LIPM) since 1986.
Representative
publications
ROBY
D., BROGLIE K., CRESSMAN R., BIDDLE P., CHET I. et BROGLIE R.,
1990. Activation of a bean chitinase promoter in transgenic tobacco
plants by phytopathogenic fungi. The Plant Cell 2, 999-1007.
GODARD
F., LUMMERZHEIM M., SAINDRENAN P., BALAGUE C. and ROBY D. (2000).
Hxc-2, an Arabidopsis mutant with altered hypersensitive response
to Xanthomonas campestris pv. campestris. Plant J. 24(6), 749-761
PONTIER
D., BALAGUE C., BEZOMBES-MARION I., TRONCHET M., DESLANDES L.
and ROBY D. (2001). Identification of a novel pathogen-responsive
element in the promoter of the tobacco gene HSR203J, a molecular
marker of the hypersensitive response. Plant J. 26(5), 495-509.
VAILLEAU
F., DANIEL X., TRONCHET M., MONTILLET JL., TRIANTAPHYLLIDES C.
and ROBY D. (2002). A R2R3-MYB gene, AtMYB30, acts as a positive
regulator of the hypersensitive cell death program in plants in
response to pathogen attack. Proc. Natl. Acad. Sci. USA 99(15),
10179-10184..
BALAGUE
C., LIN B., ALCON C., FLOTTES G., MALMSTROM S., KOHLER C., NEUHAUS
G., PELLLETIER G., GAYMARD F. and ROBY D. (2003) HLM1, an essential
component of signaling for the Hypersensitive Response, encodes
a member of the CNGC ion channel family. The Plant Cell 15(2),
365- 379.
LORRAIN
S., VAILLEAU F., BALAGUE C. and ROBY D. (2003) Lesion mimic mutants
: keys for deciphering cell death and defense pathways in plants
? Trends in Plant Sci. 8(6), 263- 271.
LORRAIN
S., LIN B., AURIAC M-C., KROJ, T., SAINDRENAN, P., NICOLE M.,
BALAGUE C. and ROBY D. (2004) VAD1, a novel GRAM domain-containing
protein, is a regulator of cell death and defense responses in
vascular tissues. The Plant Cell 16, 2217- 2232.
JOURNOT-CATALINO
N., SOMSSICH I., ROBY D. and KROJ T. (2006) The transcription
factors WRKY11 and WRKY17 act as negative regulators of basal
resistance in Arabidopsis thaliana. The Plant Cell 18, 1-14.
PERCHEPIED
L., KROJ T., TRONCHET M., LOUDET O. and ROBY D. (2006). Natural
variation in partial resistance to Pseudomonas syringae is controlled
by two major QTLs in Arabidopsis thaliana. PloS ONE 1(1): e123.
doi:10.1371/journal.pone 0000123.
RAFFAELE
S., VAILLEAU F., LEGER A., JOUBES J., MIERSCHE O., HUARD C., BLEE
E., MONGRAND S., DOMERGUE F. and ROBY D. (2008). A MYB Transcription
Factor Regulates Very Long Chain Fatty Acid Biosynthesis for Activation
of the Hypersensitive Cell Death in Arabidopsis. The Plant Cell
(sous presse)
Technological
transfer and recent contracts
Contrat
de Collaboration avec les sociétés Vilmorin Clause
& Cie, et Biogemma "Identification of new components
of resistance/cell death pathways through the search of interacting
partners of AtMYB30, a R2-R3 MYB transcriptional factor involved
in the regulation of the hypersensitive response in Arabidopsis",
2003-2006, 198,906 euros.
Trilateral co-operation between Spain (McyT), France (Genoplante)
and Germany (GABI) on Plant Genome Research "Diversity of
disease resistance mechanisms in Arabidopsis DILEMA", Coordinators
: D. Roby / J. Parker / C. Castresana, 2004-2007, 375 kE.
Contrat ANR Génoplante "Cyclic nucleotide-gated cation
channels involved in hypersensitive cell death in Arabidopsis
thaliana"(CHAT), 2006-2009.
Contrat ANR Génoplante "Identification of genes involved
in Sclerotinia sclerotiorum resistance in Oilseed Rape and Sunflower"(Sclerotinia),
2006-2009.
|
Jesús
V. JORRIN NOVO
"Titular"
Professor at the University of Córdoba. Agricultural and
Plant Biochemistry and Proteomics, Department of Biochemistry
and Molecular Biology, Agricultural and Forestry High technical
School (escuelta Técnica Superior de Ingenieros Agrónomos
y de Montes)
Address: Campus de Rabanales, Edificio Severo Ochoa (C6), planta
baja. 14071 Córdoba, Spain. Tel.: +34 957 218439; 957 218574,
Fax: +34 957218439
E-mail: bf1jonoj@uco.es
Lecture
Title:
'Proteomics in the study of plant responses to biotic and abiotic
stresses'
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|
AREAS
OF EXPERTISE/INTEREST
Agricultural
and Plant Biochemistry and Molecular Biology, Proteomics (plant
proteomics, yeast proteomics), posttranslational modifications
(redox proteome). Plant responses to biotic and abiotic stresses.
Secondary metabolism (phenolics).
GENERAL
Head
of the Agricultural and Plant Biochemistry and Proteomics, University
of Córdoba
Secretary of the Dept. of Biochemistry and Molecular Biology,
University of Córdoba (2004-2007)
Founder Member (2005) and Secretary (2005) of the Spanish Proteomics
Sciety (SEProt)
Member of the Conference and Communication Committee of the European
Proteomics Association (2006-)
Editor in Chief of "PROTEOMICA" (Journal of the Spanish
SEProt
Executive Editor of the "Journal of Proteomics"
Member of the Editorial Board of "Proteomics" and "The
Open Proteomics Journal"
Referee of: Proteomics, Phytochemistry, Physiologia Plantarum,
Plant Science, Current proteomics, Journal of Plant Physiology,
Weed Research, Journal of Heredity, Cell Research (China), ABB-Sinica,
FINNANCED
RESEARCH PROJECTS (last 5 years, 2003-2008)
Application
of proteomics to the study of sunflower, an strategic crop for
Argentina and Spain (Spanish AECI, Ministry of Foreign Affair)
(A/014378/07), 2007-2009
Plant
proteomics in Europe" EUPP (European Plant Proteomics). EU.
European Cooperation in the field of Scientific and Technical
Research (COST) (OC-2006-217; FA 0603). 2006-2011.http://www.costfa0603.com/index.php.
Gene interaction networks and models of cation homeostasis in
Saccharomyces cerevisiae. TRANSLUCENT" (EU. P-E-01-06-17).
2007-2010. http://quiro.uab.es/Jarino/Translucent/
A comparative study of the redox proteome. Junta de Andalucía,
Spain. 2007-2010.
The redox proteome: protein nitrosylation and glutathionylation
as a regulating mechanism of the plant defense responses to pathogens.
Spanish Ministry of Science and Education (DGI-MEC, Bio-2006.14790).
2006-2009.
Functional genomics (transcriptomics and proteomics) to study
variability and responses to stresses in forest tree spp. (Quercus
ilex, Quercus rubur, Pinus pinaster). Consejería de Medio
Ambiente, Junta de Andalucía. 2005-2007.
Proteomics as a novel approach to study the Q. ilex decay disease.
Monte de Piedad y Caja de ahorros de Huelva y Sevilla. 2004-2006.
New Strategies to Improve Grain Legumes for Food and Feed (EU,
FP6-506223; WP 10, biotic stresses, Proteomic approach). 2004-2007.
http://www.eugrainlegumes.org/summary/index.htm.
Resistance
mechanism to parasitic angiosperms of the genus Orobanche. Spanish
Ministry of Science and Education DGI, AGL2001-2420. 2003-2005.
Optimization
of protocols for 2-DE análisis and its application to the
proteome analysis of plant and yast (Andalusian Governement, University
and Research Secretary). 2003-2004
Parasitic
plant management in sustainable agriculture. EU COST Action 849,
2001-2006
1.
Published papers 2004-2008 ( SCI Journals)
Sira
Ehevarría-Zomeño, David Ariza, Inmaculada Jorge,
Christof Lenz, Jesús Jorrín, Rafael M Navarro Cerrillo.
2008. Changes in the protein profile of Quercus ilex leaves in
response to drought-stress and and recovery. Journal of Plant
Physiology (in press)
VALLEDOR, L., CASTILLEJO, M.A., LENZ, C., RODRÍGUEZ, R.,
CAÑAL, M.J., JORRÍN, J. 2008. The 2-DE proteome
of Pinus radiata needle tissue: analytical, biological variability
and protein identification by LC/MS/MS and substitution-tolerant
database searching. Journal of Proteome Research (in press).
CASTILLEJO
M.A., MALDONADO A..M, OGUETA S., JORRÍN, J. 2008. Proteomic
analysis of responses to drought stress in sunflower (Helianthus
annuus) by two-dimensional gel electrophoresis and mass spectrometry.
The Open Proteomics Journal (in press)
M.J.
DUNN, C. GIL, C. KLEINHAMMER, F. LOTTSPEICH, S. PENNINGTON, J.-CH.
SANCHEZ,J.P. ALBAR, L. BINI, F. CORRALES, G.L. CORTHALS, M.M.
FOUNTOULAKIS, C. HOOGLAND, P. JAMES,O.N. JENSEN, C. JIMÉNEZ,
J. JORRÍN-NOVO, H.-J. KRAUS, H. MEYER, D. NOUKAKIS,P.M.
PALAGI, D. PENQUE, A. QUINN, T. RABILLOUD. 2008. EuPA achieves
visibility - An activity report on the first three years. Journal
of Proteomics 1: 11-18.
E.
PRATS, J.C. GARCIA GALINDO, M. E. BAZZALO, A. LEÓN, F.
A. MACIAS, D. RUBIALES, J. V. JORRÍN. 2007. Antifungal
activity of constitutive phenolic compounds from capitulum of
a head rot-resistant sunflower genotype. : Journal of Chemcal
Ecology, in press ( DOI 10.1007/s10886-007-9388-9)
JORRÍN,
J., MALDONADO, A.M., CASTILEJO, MA. 2007. Plant proteome analysis:
a 2006 update. Proteomics 7 2947 -2962
PÉREZ-REINADO.E,
RAMÍREZ-BOO.M, GARRIDO.J.J, JORRÍN.J.V AND MORENO.A.
2007. Toward global analysis of porcine alveolar macrophages proteins
through two-dimensional electrophoresis and mass spectrometry.
Developmental and Comparative Immunology 31: 1220-1232
E.
PRATS, M. J. LLAMAS, J. JORRIN, AND D. RUBIALES. 2007. Constitutive
Coumarin Accumulation on Sunfl ower Leaf Surface Prevents Rust
Germ Tube Growth and Appressorium Diff erentiation. CROP SCIENCE,:
47: 1119-1124
RISPAIL.N,
DITA M.-A, GONZÁLEZ-VERDEJO.C, PÉREZ-DE-LUQUE.A,
CASTILLEJO.M.-A, PRATS.E, ROMÁN.B, JORRÍN.J, RUBIALES.D.
2007. Plant resistance to parasitic plants: molecular approaches
to an old foe. New Phytologist 173: 703-712.
FERNÁNDEZ-ACERO.F.J,
JORGE.I, CALVO.E, VALLEJO.I, CARBÚ.M, CAMAFEITA.E, GARRIDO.C,
LÓPEZ.J.A, JORRIN.J AND CANTORAL.J.M. 2007. Proteomic analysis
of phytopathogenic fungus Botrytis cinerea as a potential tool
for identifying pathogenicity factors, therapeutic targets and
for basic research. Archives of Microbiology 187: 207-215.
ROLDÁN
SERRANO.A, LUNA DEL CASTILLO.J, JORRÍN NOVO.J, FERNÁNDEZ
OCAÑA.A, GÓMEZ RODRÍGUEZ.M.V. 2007. Chitinase
and peroxidase activities in sunflower hypocotyls: effects of
BTH and inoculation with Plasmopara halstedii. Biologia Plantarum
51: 149-152.
ECHEVARRÍA-ZOMEÑO,
S., PÉREZ-DE-LUQUE, A., JORRÍN, J., MALDONADO, A.M.
2006. Pre-haustorial resistance to broomrape (Orobanche cumana)
in sunflower (Helianthus annuus): cytochemical studies. Journal
of Experimental Botany, 57:4189-420
ROSSIGNOL.M,
PELTIER.JB, MOCK.H.P, MATROS.A, MALDONADO.A, JORRÍN.J.
2006. Plant proteome analysis: a 2004-2006 update. Proteomics
6:5529-5548.
BÁRCENA
JA, CALVETE JJ, MORENO-LOPEZ A, JORRÍN-NOVO JV. 2006. Editorial:
out of Córdoba. Proteomics S1: 1-3-
JORGE
I, NAVARRO, RM, LENZ C, ARIZA D, JORRÍN J. 2006. Variation
in the holm oak leaf proteome at different plant developmental
stages, between provenances and in response to drought stress.
Proteomics S1´06: 207-214.
FERNÁNDEZ-ACERO
FJ, JORGE I, CALVO E, VALLEJO I, CAMAFEITA LE, LÓPEZ JA,
CANTORAL JM, JORRIN J. 2006. Two-dimensional electrophoresis protein
profile of the phytopathogenic fungus Botrytis cinerea. Proteomics
S1´06: 88-96.
CURTO,
M., CAMAFEITA, E., LOPEZ, J.A., MALDONADO, A.M., RUBIALES, D.,
JORRÍN, J.V. 2006. A proteomic approach to study pea (Pisum
sativum) responses to powdery mildew (Erysiphe pisi). Proteomics
S1´06: 163-174.
JORRÍN,
J.V., RUBIALES, D., DUMAS-GAUDOT, E., RECORBET, G., MALDONADO,
A., CASTILLEJO, M.A., CURTO, M. 2006. Proteomics: a promising
approach to study biotic stresses in legumes. A review. Euphytica
147: 37-47.
PRATS.E.,
BAZALO.M.E., JORRÍN.J.V. 2006. Fungitoxic effect of scopolin
and related coumarins on Sclerotinia sclerotiorum. A way to overcome
sunflower head rot. Euphytica 147: 451-460.
JORRÍN.J.,
BÁRCENA.J.A., CALVETE.J.J. 2005. Report on the First Congress
of the Spanish Proteomics Society. Proteomics 5: 2712-2715.
PÉREZ
DE LUQUE.A., JORRÍN.J.V., CUBERO.J.I, RUBIALES, D. 2005.
Orobanche crenata resistance and avoidance in pea (Pisum spp.)
operate at different developmental stages of the parasite. Weed
Science 45: 379-387.
JORGE,
I., NAVARRO, M., LENZ, C., ARIZA, D., PORRAS, C., JORRÍN,
J. 2005. . The Holm Oak leaf proteome. Analytical and biological
variability in the protein expression level assessed by 2-DE and
protein identification by MS/MS de novo sequencing and sequence
similarity searching. Proteomics 5: 222-234.
CÁNOVAS.M,
DUMAS-GAUDOT, E., RECORBET, G., JORRÍN, J., MOCK, H.P.,
ROSSIGNOL, M. 2004. Plant proteome analysis. Proteomics 4: 285-298.
CASTILLEJO,
M.A., AMIOUR, N., DUMAS-GAUDOT, E., RUBIALES, D., JORRÍN,
J.V. 2004. A proteomic approach to studying plant response to
crenate broomrape (Orobanche crenata) in pea (Pisum sativum).
Phytochemistry 65: 1817-1828.
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Prof.
Ramón Serrano
Instituto
de Biología Molecular y Celular de Plantas (IBMCP), Ciudad
Politécnica de la Innovación, Universidad Politécnica
de Valencia-CSIC, Camino de Vera s/n, 46022 Valencia, Spain.
Tel. 34-96-3877883;
Fax 34-96-3877859
e-mail: rserrano@ibmcp.upv.es
Lecture
Title:
'Probing basic cellular functions by abiotic stress' |
|
Ramón
Serrano is born at Valencia, Spain. He is Doctor Agricultural
Enginneer, Research Professor of the spanish CSIC (Research Council)
and Full Professor of Biochemistry and Molecular Biology at the
Polytechnic University of Valencia, Spain.
Condecorated
with the "Cruz de Alfonso X El Sabio" of the spanish
government and Doctor Honoris Causa by the University of Regensburg,
Germany.
The scientific background of Ramon Serrano is based on the training
in the laboratories of three master scientists of modern biology:
the spanish enzymologist Alberto Sols, the american bioenergeticist
Efraim Racker and the american molecular biologists Gerald R.
Fink. Their influence generated an interest at the molecular level
on the connection between membrane transport, enzyme activity
and cell growth.
The first achievement of Ramon Serrano as independent scientist
was the characterization of the proton pump of yeast and plants.
In animal cells, Jens C. Skou (Nobel Prize of Chemistry in 1997)
discovered in the late fifties an ATPase which coupled ATP hydrolysis
to Na and K transport across the plasma membrane. However, this
enzyme does not exist in fungi and plants and the bioenergetics
of the plasma membrane of non-animal cells was plunged into confusion
for many years. Thanks to the work of Ramon Serrano in Spain,
together with that of André Goffeau in Belgium and of Carolyn
W. Slayman in USA, it became clear that the plasma membrane of
fungi and plants is energized by a proton-pumping ATPase, not
by a Na-K dependent enzyme.
Ramon Serrano was also at the frontline in the molecular cloning
of the gene of the yeast proton pump, work performed in collaboration
with Gerald R. Fink at the Whitehead Institute (MIT, Cambridge,
Massachussetts, USA). Also, the plant proton pump was cloned for
the first time in his laboratory at Heidelberg at the same time
than in the laboratory of M.R. Sussman at Madison (USA). These
non-animal H-ATPases belonged to the same protein family than
the Na-K ATPase. Apparently, in the course of evolution, a primaeval
pump had diverged into the animal and non-animal lineages. Animal
cells have a primary sodium pump to regulate its volume and avoid
swelling. On the other hand, fungal and plant cells obviate this
problem by the presence of a rigid cell wall and exploit proton
pumping.
Further work of Ramon Serrano defined the essential residues of
the proton pump and identified a regulatory domain at its carboxyl
terminus as well as a phosphatase domain essential to complete
the catalytic cycle by hydrolysis of the phosphorylated intermediate.
From the physiological point of view, he demonstrated a connection
between cell growth and proton pump activity in yeast. Active
nutrient transport and pH regulation were implicated in this important
phenomenon. In order to extrapolate these results to animal cells,
the yeast proton pump was expressed in fibroblast, which experienced
elevated intracellular pH and increased proliferation (in collaboration
with Rosario Perona, Madrid). In plants, the effect of auxin on
cell growth was correlated with induced expression of the proton
pump (in collaboration with Achim Hager, Tubingen).
Since 1990, Ramon Serrano started to work on the molecular basis
of proton, potassium and sodium homeostasis, utilizing the yeast
Saccharomyces cerevisiae and the plant Arabidopsis thaliana as
model system. This was another aspect of the connection between
membrane transport and cell growth and had the social relevance
that it could generate the biotechnological tools to improve the
salt tolerance of crops, an urgent need for the future of agriculture
in irrigated land. This work identified the first target of sodium
toxicity in living cells, the nucleotidase encoded by the yeast
HAL2 gene. This enzyme is very sensitive to inhibition by Li and
Na and it is required to detoxify a metabolite, adenosine-3',5'-diphosphate
or PAP, which inhibits sulfate assimilation, sulfate transfer
and RNA processing. Plant HAL2 homologues were characterized which
could be targets of sodium toxicity. The Hal2 protein has been
crystallized and the molecular basis of Li and Na inhibition characterized
at the atomic level (in collaboration with Armando Albert at Madrid
and Tom Blundel at Cambridge).
The transcription factor Sko1 has been identified by his laboratory
as a repressor of some defense genes modulated by the yeast MAP
kinase Hog1, which is activated by salt stress. This established
a novel paradigm of negative control for salt stress defense genes
such as ENA1 (encoding a Na+ extrussion pump) and HAL1 (encoding
an activator of K+ transport). The identification of protein kinase
(HAL4 and HAL5) and protein phosphatase (PPZ1) genes as modulators
of K+ transport opened the molecular bases of ion homeostasis
in yeast. Ppz1 is inhibited by Hal3 and the Hal3-Ppz1 interaction
is pH-dependent, constituing the first pH sensors identified in
cells.
Latest works of Ramón Serrano have established a connection
between plant sulphydryl oxidases and ion homeostasis through
regulation of potassium transport at the xylem loading interface.
Also, oxidative stress has been found to desensitize ethylene
responses in Arabidopsis.
Main
publications last 5 years
Rausell,
A., Kanhonou, R., Yenush, L., Serrano, R. and Ros, R.
The translation initiation factor eIF1A is an important determinant
in the tolerance to NaCl stress in yeast and plants
Plant J. 34 (2003) 257-267
Naranjo,
M.A., Romero, C., Bellés, J.M., Montesinos, C., Vicente,
O. and Serrano, R.
Lithium treatment induces a hypersensitive-like response in tobacco
Planta 217 (2003) 417-424.
Mulet,
J.M., Alemany, B., Ros, R., Calvete, J.J. and Serrano, R.
Expression of a plant serine O-acetyltransferase in Saccharomyces
cerevisiae confers osmotic tolerance and creates an alternative
pathway for cysteine biosynthesis
Yeast 21 (2004) 303-312
Mulet,
J.M., Alejandro, S., Romero, C. and Serrano, R.
The trehalose pathway and intracellular glucose phosphates as
modulators of potassium transport and general cation homeostasis
in yeast
Yeast 21 (2004) 569-582
Gonzalez-Guzmán. M., Abia, D., Salinas, J., Serrano, R.
and Rodriguez, P.L.
Two new alleles of the abscisic aldehyde oxidase 3 gene reveal
its role in abscisic acid
biosynthesis in seeds
Plant Physiol. 135 (2004) 325-333
Merchan,
S., Bernal, D., Serrano, R. And Yenush, L.
Response of the Saccharomyces cerevisiae Mpk1 mitogen-activated
protein kinase pathway to increases in internal turgor pressure
caused by loss of Ppz protein phosphatases
Eukar. Cell 3 (2004) 100-107
Portillo
F, Mulet JM, Serrano R
A role for the non-phosphorylated form of yeast Snf1: tolerance
to toxic
cations and activation of potassium transport.'
FEBS Lett. 579 (2005) 512-516
Forment,J.,
Gadea,J., Huerta,L., Abizanda,L., Agusti,J., Alamar,S., Alos,E.,
Andres,F.,
Beltran,J.P., Berbel,A., Blazquez,M.A1, Brumos,J., Canas,L.A.,
Cercos,M., Colmenero-Flores,J.M., Conesa,A, Estables,B, Gandia,M.,
Garcia-Martinez,J.L, Gimeno,J., Gisbert,A., Gomez,G., Gonzalez-Candelas,L.,
Granell,A., Guerri,J., Lafuente,M.T. Madueno,F, Marcos,J.F., Martinez,F.,
Martinez-Godoy,M.A., Miralles,S., Moreno,P., Navarro,L, Pallas,V,
Perez-Amador,M.A., Perez-Valle,J., Pons,C., Rodrigo,I., Rodriguez,P.L.,
Royo,C., Serrano,R., Soler,G., Tadeo,F., Talon,M., Terol,J., Trenor,M,
Vaello,L, Vicente,O, Vidal,Ch., Zacarias,L. and Conejero,V.
Development of a citrus genome-wide EST collection and cDNA microarray
as resources for genomic studies
Plant. Mol. Biol. 57 (2005) 375-391
Yenush,
L., Merchan S, Holmes J, and Serrano R.
pH-responsive, post-translational regulation of the Trk1 potassium
transporter by the type I-related Ppz1 phosphatase
Mol. Cell. Biol. 25 (2005) 8683-8692
Rubio,
S., Larson, T.R., Gonzalez-Guzman, M., Alejandro, S., Graham,
I.A., Serrano, R.
and Rodriguez, P.L. .
An Arabidopsis mutant impaired in CoA biosynthesis is sugar-dependent
for seedling establishment
Plant Physiol. 140 (2006) 830-843
Naranjo
MA, Forment J, Roldan M, Serrano R, Vicente O
Overexpression of Arabidopsis thaliana LTL1, a salt-induced gene
encoding
a GDSL-motif lipase, increases salt tolerance in yeast and transgenic
plants.
Plant Cell Environ. 29 (2006) 1890-900
Alejandro
S, Rodriguez PL, Bellés JM, Yenush L, Garcia-Sanchez MJ,
Fernandez JA, Serrano R
An Arabidopsis quiescin-sulphydryl oxidase regulates cation homeostasis
at the root-symplast interface
EMBO J. 26 (2007) 3203-3215
Perez-Valle J, Jenkins H, Merchan S, Montiel V, Ramos J, Sharma
S, Serrano R,Yenush L.
A key role for intracellular K+ and protein kinases Sat4/Hal4
and Hal5 in the plasma membrane stabilization of yeast nutrient
transporters.
Mol. Cell. Biol. 27 (2007) 5725-5736
Bueso,
E., Alejandro, S., Carbonell, P., Perez-Amador, M.A., Fayos, J.,
Bellés, J.M., Rodriguez, P.L. and Serrano, R.
The lithium tolderance of the Arabidopsis cat2 mutant reveals
a cross-talk between oxidative stress and ethylene
Plant J. 52 (2007) 1052-1065
|
Jian-Kang
ZHU
Department
of Botany and Plant Sciences. Institute for Integrative Genome
Biology 2150 Batchelor Hall, University of California, USA
Riverside, CA 9252, Tel. 1 951 827 7117, Fax 1 951 827 7115
Email: jian-kang.zhu@ucr.edu
Lecture
Title: Small RNAs and epigenetic regulation
in plant abiotic stress responses.
|
|
Education
Beijing Agricultural Univ., China Soils and Agri. Chem. B.S. 1987
University of California, Riverside, CA Botany M.S. 1990
Purdue University, West Lafayette, IN Plant Physiology Ph.D. 1993
Professional
Experience
2004-Present Jane Johnson Chair and Professor, Botany and Plant
Sciences, University of California, Riverside
2004-2006 Director, Institute for Integrative Genome Biology,
UC Riverside
2000-2003 Professor, Dept. of Plant Sciences, University of Arizona
1999 Associate Professor, Dept. of Plant Sciences, University
of Arizona
1996-1998 Assistant Professor, Dept. of Plant Sciences, University
of Arizona
1995 Assistant Professor, Dept. of Botany & Microbiology,
Auburn University
1994 Postdoctoral Fellow, Laboratory of Plant Molecular Biology,
Rockefeller University
Publications
(i) Publications most closely related to proposed project
" Jin, H., Vacic, V., Girke, T., Lonardi, S., Zhu, J.K. 2008,
Small RNAs and the regulation of cis-natural antisense transcripts
in Arabidopsis. BMC Mol. Biol. 9: 6
" Borsani, O., Zhu, J., Verslues, P.E., Zhu, J.K. 2005. A
novel biogenesis pathway and physiological function for endogenous
siRNAs from natural cis-antisense transcripts in Arabidopsis.
Cell 123: 1279-1291.
" Sunkar, R., Girke, T., Zhu, J.K. 2005. Identification and
characterization of endogenous small-interfering RNAs from rice.
Nucleic Acids Res. 33: 4443-4454.
" Sunkar, R., Girke, T., Jain, P.K., Zhu, J.K. 2005. Cloning
and Characterization of microRNAs from Rice. Plant Cell 17: 1397-1411.
" Sunkar, R., Zhu J.K. 2004. Novel and stress regulated miRNAs
and other small RNAs from Arabidopsis. Plant Cell 16: 2001-2019.
(ii) Other significant publications
" Sridhar, V.V., Kapoor, A., Zhang, K., Zhu, J., Zhou, T.,
Hasegawa, P.M., Bressan, R.A., Zhu, J.K. 2007. Control of DNA
methylation and heterochromatic silencing by histone H2B deubiquitination.
Nature. 447: 735-738.
" Sunkar, R., Kapoor, A., Zhu, J.K. 2006. Posttranscriptional
induction of two cu/zn superoxide dismutase genes in Arabidopsis
is mediated by downregulation of mir398 and important for oxidative
stress tolerance. Plant Cell 18: 2051-2065.
" Gong, Z., Morales-Ruiz, T., Ariza, R.R., Roldán-Arjona,
T., David, L. and Zhu, J.-K. 2002. ROS1, a repressor of transcriptional
gene silencing in Arabidopsis, encodes a DNA glycosylase/lyase.
Cell 111: 803-814.
" Kapoor, A., Agarwal, M., Andreucci, A., Zheng, X., Gong,
Z., Hasegawa, P.M., Bressan, R.A., Zhu, J.K. 2005. Loss-of-function
mutations in a conserved replication protien suppress transcriptional
gene silencing in a DNA methylation-independent manner in Arabidopsis.
Current Biol. 15: 1912-1918.
" Fujii, H., Chiou, T.J., Lin, S.I., Aung, K., Zhu, J.K.
2005. A miRNA involved in phosphate-starvation response in Arabidopsis.
Curr Biol. 15: 2038-2043.
" Yao Y, Guo G, Ni Z, Sunkar R, Du J, Zhu JK, Sun Q. 2007.
Cloning and characterization of microRNAs from wheat (Triticum
aestivum L.). Genome Biol. 8: R96
Synergistic
Activities
Associate editor, Plant Physiology (2004-2005); Monitoring editor,
Plant Physiology (2000-2003); Associate editor, Plant Molecular
Biology (2000-present);Associate editor, Molecular, Genetics and
Genomics (2005-present); Editorial board, Integrative Plant Biology
(2001-); Editorial advisory board, Plant Journal (2001-); ASPB
international committee, (1999-2001); International review committee
and advisory board member, Institute of Bioagricultural Sciences,
Academia Sinica, Taiwan (2000-); Panel member, USDA NRICGP, NSF,
DOE; Conference/session organizer/chair, e.g. Gordon Conference
on Temperature Stress (2005), International Workshop on Drought
Tolerance, Jinan, China (2002), International Conference on Arabidopsis
Research (1998; 2002, 2004), ASPP Annual Meeting (1998; 2002),
NATO workshop on plant stress tolerance, Poland (1999).
Collaborators
(i) Collaborators:
John Cushman, University of Nevada, Reno
Julia Bailey-Serres, University of California, Riverside
Thomas Girke, University of California, Riverside
Pamela Ronald, University of California, Davis
Ron Mittler, Unviversity of Nevada, Reno
Martin Gollery, Unviversity of Nevada, Reno
Jeffrey Harper, Unviversity of Nevada, Reno
Michael Fromm, University of Nebraska, Lincoln
Wenyuan Song, University of Florida
(ii) Graduate and Postdoctoral Advisors:
Dr. Nam-Hai Chua (postdoctoral advisor), The Rockefeller University
Dr. Paul M. Hasegawa (Ph.D. advisor), Purdue University
(iii) Thesis Advisor and Postgraduate-Scholar Sponsor:
Dr. Deming Gong, HortResearch New Zealand
Dr. Yan Guo, China
Dr. Zhizhong Gong, China
Dr. Manabu Ishitani, Columbia
Dr. Cheol-Soo Kim, Korea
Yongsig Kim, University of Arizona
Byeongha Lee, University of Arizona
Dr. Hojoung Lee, Korea University
Dr. Jiping Liu, Cornell University
Dr. Masaru Ohta, University of Tsukuba
Dr. Huazhong Shi, Texas Tech
Dr. Liming Xiong, Danforth Plant Science Center
Dr. Monkia Mikolajczyk, The University of Arizona
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Vicente
RUBIO
Research
Professor of the Spanish Research Council (CSIC)
Director of the Instituto de Biomedicina of Valencia (IBV-CSIC)
Instituto de Biomedicina de Valencia, Jaume Roig 11, Valencia-46010,
Spain
Phone +34 96 3391772; Fax +34 96 3690800; E-mail: rubio@ibv.csic.es
Lecture
Title: Arginine and Nitrogen Storage:
a Structural View
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Professor,
Doctoral Program, Biochemistry Dept., University of Valencia
President, Spanish Society for Biochemistry and Molecular Biology
(SEBBM)(09/2004-09/2008)
Head, group 39, Center for Biomedical Networked Research on Rare
Diseases (CIBERER)
Education
University of Valencia Medical School M.D. (1972)
University of Valencia Medical School Ph.D. (1976) Biochemistry
Kansas University Medical Center Postdoc. (02/1974-12/1976) Biochemistry
Spanish Ministry of Education Medical Specialty (1980) Clinical
Chemistry
Research
and professional experience
1972-74. Fellow of the Instituto de Investigaciones Citológicas,
Valencia.
1974. Spanish Ministry of Science Fellow, Dept. of Biochemistry,
Kansas University Medical Center, Kansas City. Research director:
Dr. Santiago Grisolia.
1975-76. Research Associate, Biochemistry Dept., Kansas University
Medical Center, Kansas City.
1977-78. Staff Researcher, Cell Chemistry Dept., Instituto de
Investigaciones Citológicas, Valencia.
1978-1998. Head, Cell Chemistry Dept., Inst. Investigaciones Citológicas,
Valencia.
1981-1998. Research Professor, International Molecular Cytology
Program, University of Kansas.
1982-87. Assistant Professor of Biochemistry, University of Valencia.
1987-present. Professor Ph.D. Programme, Dept. of Biochemistry,
University of Valencia.
1987 2003. Investigador, Consejo Superior de Investigaciones Científicas
(CSIC, Spanish Science Research Council).
1996-present Director, Instituto de Biomedicina de Valencia CSIC),
and Head of the Molecular Enzymology and Enzymopathology Laboratory
1996-1999. Member of the Executive Board, Spanish Society of Biochemistry
and Molecular Biology (SEBBM).
2002-2004 President Elect of SEBBM.
2004-present Profesor de Investigación (Research Professor)
Consejo Superior de Investigaciones Científicas (CSIC,
Spanish Science Research Council).
2002-2006 Professor, Severo Ochoa Molecular Medicine Chair, (CSIC-Santander
Central Hispano) held each year in a Latin-American University.
2004-2008 President, Spanish Society of Biochemistry and Molecular
Biology (SEBBM).
Appointments,
Grants and Awards. Has been or is a member of Advisory Scientific
Committees of the Fundación Valenciana de Investigaciones
Biomedicas, of the Fundación de Investigación del
Hospital "La Fe" (Valencia), and of Spanish granting
bodies (Ministries of Health and of Education and Science), a
Spanish representative to the standing Committee of the European
Medical Research Council (EMRC, the medical branch of ESF), ad
hoc grant reviewer for the NSF (USA) and ANEP (Spain), manuscript
reviewer for several international scientific journals. Head investigator
in 24 grants for research. Art/Sciences 2001 Award of the Valencian
Community, and Cátedra Bernal Castejón Of the University
of Zaragoza (2007).
Research
interests and activity. His research is centered on studies of
the structure and function of enzymes of biomedical interest,
and of their complexes, having created a protein crystallography
unit in Valencia. He also studies the genetic and molecular bases
of inborn errors, particularly those affecting the urea cycle.
Author of over 100 research papers and 7 reviews in international
journals, coauthor of 14 books, co-organizer of 10 scientific
meetings, having had top responsibility or co-responsibility for
the organization of 3 international meetings and two Spanishs
meetings.
Ten
recent publications
Llacer, J.L., Fita, I., Rubio, V. (2008) Arginine and Nitrogen
Storage. Curr. Opin. Struc. Biol., in press.
Fernández-Murga, M.L., Rubio, V. (2008) The basis of the
arginine sensitivity of microbial N-acetyl-L-glutamate kinases:
a mutagenesis and protein engineering study with the Pseudomonas
aeruginosa and Escherichia coli enzymes. J. Bacteriol. 190:3018-25
Llácer, J.L., Contreras, A., Forchhammer, K., Marco-Marin,
C., Gil-Ortiz, F., Maldonado, F., Fita, I., Rubio, V. (2007) The
crystal structure of the complex of PII and acetylglutamate kinase
reveals how PII controls the storage of nitrogen as arginine.
Proc. Natl. Acad. Sci. USA 104:17644-17649.
Marco-Marin, C., Gil-Ortiz, F., Perez-Arellano, I., Cervera, J.,
Fita, I., Rubio, V. (2007) A novel two-domain architecture within
the amino acid kinase enzyme family revealed by the crystal structure
of Escherichia coli glutamate 5-kinase. J. Mol. Biol. (2007) 367:1431-1446.
Llácer, J.L., Polo, L.M., Tavarez, S., Alarcon, B., Hilario,
R., Rubio, V. (2007) The gene cluster for agmatine catabolism
of Enterococcus faecalis. Studies of recombinant putrescine transcarbamylase
and agmatine deiminase and a snapshot of agmatine deiminase catalyzing
its reaction. J Bacteriol 189:1254-1265.
Ramón-Maiques, S., Fernández-Murga, M.L., Gil-Ortiz,
F., Vagin, A., Fita, I., Rubio, V. (2006) Structural bases of
feed-back control of arginine biosynthesis, revealed by the structures
of two hexameric N-acetylglutamate kinases, from Thermotoga maritima
and Pseudomonas aeruginosa. J. Mol. Biol. 356:695-713.
Marco-Marin, C., Gil-Ortiz, F., Rubio, V. (2005) The crystal structure
of Pyrococcus furiosus UMP kinase provides insight into catalysis
and regulation in microbial pyrimidine nucleotide biosynthesis.
J. Mol. Biol. 352: 438-454.
Yefimenko, I., Fresquet, V., Marco-Marín, C., Rubio, V.
Cervera, J. (2005) Understanding carbamoyl phosphate synthetase
deficiency: impact of clinical mutations on enzyme functionality.
J Mol Biol 349:127-141.
Fernández-Murga, M.L., Gil-Ortiz, F., Llácer, J.L.,
Rubio, V. (2004) Arginine biosynthesis in Thermotoga maritima.
Characterization of the arginine-sensitive N-acetyl-L-glutamate
kinase. J. Bacteriol. 186, 6142-6149.
Marco-Marín, C., Ramon-Maiques, S., Tavárez, S.,
Rubio, V. (2003) Site-directed mutagenesis of Escherichia coli
acetylglutamate kinase and aspartokinase III probes the catalytic
and substrate-binding mechanisms of these amino acid kinase family
enzymes and allows three-dimensional modelling of aspartokinase.J
Mol Biol 334, 459-476.
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Mohammed
BAAZIZ
Professor,
Cadi Ayyad University, Marrakech, Morocco. former President of
the Moroccan Society of Biochemistry and Molecular Biology (SMBBM)
during the last period 2004-2007, Head of the Laboratory of Biochemistry
and Plant Biotechnology, Faculty of Sciences-Semlalia, P.O.Box
2390, 40000 Marrakech, Morocco.
E-mail:
Lecture
Title:
'Peroxidases and their involvement in plant resistance to stresses.
Case of date palm fusariosis (Bayoud disease)'
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Scientific
advisory Committee:
-
1998 until 2006: Scientific Adviser for the International Foundation
for Sciences (IFS, Stockholm, Sweden).
- 1999 until 2006 : Expert for the National Commission 'Commission
Nationale d'Accréditation et d'Evaluation' (CNAE),
- 1999-2000: Member of the National Commission 'Commission Nationale
d'Evaluation des Programmes Thématiques d'Appui à
la Recherche Scientifique' (PROTARS),
Awards
-
1999: IFS Silver Jubilee Award, by the International Foundation
for Sciences (IFS, Stockholm, Sweden.
Titles
2003: Cabaliero Del Palme by San Remo city, Italy.
Reviewer
- Cahiers de la recherche, Université Hassan II-Casablanca,
Maroc.
- Annals of Botany, Oxford University Press, ISSN: 0305-7364.
- Scientia Horticulturae, Elsevier ISSN: 0304-4238, depuis Mai
2007.
- The Open Horticulture Journal, Bentham Open Access ISSN: 1874-8406
Member
of the editorial board:
- 'The
Open Horticulture Journal', Bentham Open Access ISSN: 1874-8406
Publications
for the last 5 years:
-
BOUDA, S., DEL CAMPO, F., HADDIOUI, A., BAAZIZ, M. & HERNÁNDEZ,
E.E. 2008. RAPD and ITS-based variability revealed in Atriplex
species introduced to semi-arid zones of Morocco. Scientia Horticulturae
118, 172-179.
-MRABET,
A., FERCHICHI, A., CHAIRA, N., BEN SALAH, M., BAAZIZ, M. &
MRABET PENNY, T. 2008. Physico-chemical characteristics and
total quality of date palm varieties grown in the southern of
Tunisia. Pakistan Journal of Biological Sciences 11, 1003-1008.
- QACIF,
N., BAAZIZ, M. & BENDIAB, K. 2007. Biochemical investigations
on peroxidase contents of male and female inflorescences of date
palm (Phoenix dactylifera L.). Scientia Horticulturae 114,
298-301.
- HADDIOUI,
A. & BAAZIZ, M. 2006. Effect of salinity on seed germination
and early growth of five natural populations of Atriplex halimus
L. in Morocco ; Physiol. Mol. Biol. Plants 12, 247-251.
-KHALES,
A. & BAAZIZ, M. 2005. Quantitative and qualitative aspects
of peroxidases extracted from cladodes of Opuntia ficus indica.
Scientia horticulturae 103, 209-218.
-TAHLIL,
N., RADA, A., EL GHARMALI, A., BAAZIZ, M. & EL MERAY, M. 2003.
Use of peroxidase activity in leaves and roots of two varieties
of zucchini (Cucurbita pepo L.) as biomarkers of toxicity
of soil contaminated by the spread of urban wastes. Agrochimica
XLVII (3-4), 241-247.
-AZEQOUR,
M., AMSSA, M. & BAAZIZ, M. 2002. Identification de la
variabilité intra-clonale des vitroplants de palmier dattier
issus de culture in vitro par organogenèse. Etude morphologique.
C. R. Biologies 325, 947-956.
-MAJOURHAT,
K., BENDIAB, K., MEDRAOUI, L. & BAAZIZ, M. 2002. Diversity
of leaf peroxidases in date palm (Phoenix dactylifera L.)
as revealed in an example of marginal (seedling-derived) palm
groves. Scientia Horticulturae 95, 31-38.
-AZEQOUR,
M., MAJOURHAT, K. & BAAZIZ, M. 2002. Morphological variations
and isoenzyme polymorphism of date palm clones from in vitro culture
acclimatized and established on soil in south Morocoo. Euphytica
123, 57-66.
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Jacqueline
C. BEDE
Assistant
Professor, Department of Plant Science, McGill University, Canada
E-mail: jacqueline.bede@mcgill.ca
Lecture
Title:
'Plant-Insect Interactions: the Potential Role of Cross-Talk in
Caterpillar-Mediated Circumvention of Plant Defense Responses'
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Research:
Plants adjust their defense responses to target herbivores. In response,
insect herbivores have evolved adaptive strategies to undermine
these plant defenses. Research in Bede's laboratory lies at the
boundary of plant-caterpillar interactions, characterizing the co-adaptive
responses between plant and caterpillar herbivores at the molecular,
biochemical and physiological levels. Understanding the underpinnings
of these complex interactions is critical to gain insight into the
complex molecular evolution between plants and insects and develop
effective pest management strategies.
Selected
publications:
Weech, MH, Chapleau, M, Pan, L, Ide, C and Bede, JC. (2008) Caterpillar
saliva interferes with induced Arabidopsis thaliana defense responses
via the systemic acquired resistance pathway. Journal of Experimental
Botany. 59: 2437-2448
Babic,
B, Poisson, A, Darwish, S, Lacasse, J, Merkx-Jacques, M, Despland,
E and Bede, JC. 2008. Influence of dietary nutritional composition
on caterpillar salivary enzyme activity. Journal of Insect Physiology.
54: 286-296.
Merkx-Jacques,
M, Despland, E and Bede, JC. 2008. Nutrient utilization by generalist
caterpillars of the beet armyworm, Spodoptera exigua. Physiological
Entomology. 33: 51-61.
Darwish,
S, Pan, L, Ide, C, Bede JC. 2008. Caterpillar-specific gene expression
in Medicago trucatula. Plant Molecular Biology Reporter 26: 12-31.
Bede,
JC, McNeil, JN, Tobe, SS. 2007. The role of neuropeptides in caterpillar
nutritional ecology. Peptides 28:185-186
Bede,
JC, Musser, RO, Felton, GW and Korth, KL. 2006. Caterpillar herbivory
and salivary enzymes decrease transcript levels of Medicago truncatula
genes encoding early enzymes in terpenoid biosynthesis. Plant
Molecular Biology 60: 519-530
Merkx-Jacques,
M and Bede, JC. 2005. Influence of diet on larval Spodoptera exigua
glucose oxidase activity. Journal Insect Science 5: 48.
Gomez,
SK, Cox, MM, Bede, JC, Inoue, K, Alborn, HT, Tumlinson, JH and
Korth, KL 2005. Lepidopteran herbivory and oral factors induce
transcripts encoding novel terpene synthases in Medicago truncatula.
Arch. Insect Biochem. Physiol. 58:114-127.
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Joaquin
ARIÑO
"Catedrático"
(Full Professor), Dept. Biochemistry and Molecular Biology, Autonomous
University of Barcelona. Dept. Bioquímica, Fac. Veterinaria,
Universitat Autònoma de Barcelona, Bellaterra, Barcelona
08193, Spain
Phone: 34-93-5812182, FAX 34-93-5812006, e-mail: Joaquin.Arino@uab.es
Lecture
Title: 'Unraveling cation homoestasis
in yeast: a model for plant stress adaptation'
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1986.
Ph.D. in Biochemistry (University of Barcelona)
Academic
Positions
1986-1987. Post-doctoral Fellow (Fulbright-MEC Fellowship), University
of Massachusetts Medical School.
1988-1993. "Profesor Titular" (Assistant Professor),
Dept. Biochemistry and Molecular Biology, Autonomous University
of Barcelona.
1993-to date. "Catedrático" (Full Professor),
Dept. Biochemistry and Molecular Biology, Autonomous University
of Barcelona.
Research
interests
1.- Regulation
of biological processes by phospho-dephosphorylation mechanisms
(mainly Ser/Thr protein phosphatases)
2.- Response
to stress in yeast (saline, osmotic, oxidative, alkaline).
3.- Gene regulation
and genome-wide transcriptional analysis.
Publications
(Last
5 years)
(Around
90 articles or reviews in international research journals. Publication
listed below correspond to those appeared in the last 5 years)
-
Ruiz, A., Serrano, R., & Ariño, J. (2008) Direct regulation
of genes involved in glucose utilization by the calcium/calcineurin
pathway. J. Biol. Chem. 283, 13923-33.
-
Moreno-Romero, J., Espunya, M. C. Platara, M., Ariño, J.
& Martínez M. C. (2008) A role for protein kinase CK2
in cell division in the context of Arabidopsis thaliana development:
evidence using a dominant negative mutant. Plant Journal 55, 118-130.
-
González, A., Larroy, C., Biosca, J.A. & Ariño,
J. (2008) The use of the TRP1 auxotrophic marker for gene disruption
and phenotypic analysis in yeast: a note of warning. FEMS Yeast
Research 8, 2-5.
-
Menacho-Marquez, M., Perez-Valle, J. Ariño, J., Gadea,
J. & Murguia JR (2007) Gcn2p regulates a G1/S cell cycle checkpoint
in response to DNA damage. Cell Cycle 6, 2302 - 2305.
-
Pagani, M.A., Casamayor, A., Serrano, R., Atrian, S. & Ariño,
J. (2007) Disruption of iron homeostasis in Saccharomyces cerevisiae
by high zinc levels: a genome-wide study. Mol. Microbiol. 65,
521-537.
-
Aksenova, A., Muñoz, I., Volkov, K., Ariño, J. &
Mironova, L. (2007) The Hal3-Ppz1 dependent regulation of nonsense
suppression efficiency in yeast and its influence on manifestation
of the yeast prion-like determinant [isp+]. Genes to Cells, 12
435-445.
-
Pedelini, L., Marquina, M., Ariño, J., Casamayor, A., Sanz,
L., Bollen, M., Sanz, P. & Garcia-Gimeno, M.A. (2007) Ypi1
and Sds22 regulate the nuclear localization and function of yeast
type 1 phosphatase Glc7. J. Biol. Chem. 282, 3282-3292.
-
Serrano, R., Martín, H., Casamayor, A. & Ariño,
J. (2006) Signalling alkaline pH stress in the yeast Saccharomyces
cerevisiae through the Wsc1 cell surface sensor and the Slt2 MAP
kinase pathway. J. Biol. Chem. 281, 39785-39795.
-
Platara, M., Ruiz, A., Serrano, R., Palomino, A., Moreno, F. &
Ariño, J. (2006). The transcriptional response of the yeast
Na+-ATPase ENA1 gene to alkaline stress involves three main signaling
pathways. J. Biol. Chem. 281, 36632-36642.
-
González, A., Ruiz, A., Serrano, R., Ariño, J. &
Casamayor, A. (2006) Transcriptional profiling of the protein
phosphatase 2C family in yeast provides insights into the unique
functional roles of Ptc1. J. Biol. Chem. 281, 35057-35069.
-
Ruiz, A., González, A., García-Salcedo, R., Ramos,
J. & Ariño, J. (2006) Role of protein phosphatases
2C on tolerance to lithium toxicity in the yeast Saccharomyces
cerevisiae. Mol. Microbiol. 62, 263-277.
-
García-Salcedo, R., Casamayor, A., Ruiz, A., González,
A., Prista, C., Loureiro-Dias, M.C., Ramos, J. & Ariño,
J. (2006) Heterologous expression implicates a GATA factor in
regulation of nitrogen metabolic genes and ion homeostasis in
the halotolerant yeast Debaryomyces hansenii. Eukaryotic Cell.
5, 1388-1398.
-
Ruiz, A., Ruiz, M.A., Sánchez-Garrido, M.A., Ariño,
J. & Ramos, J. (2004) The Ppz protein phosphatases regulate
Trk-independent potassium influx in yeast. FEBS Lett. 578, 58-62.
-
Muñoz, I., Ruiz, A., Marquina, M., Barceló, A.,
Albert, A., & Ariño, J. (2004) Functional Characterization
of the Yeast Ppz1 Phosphatase Inhibitory Subunit Hal3. A Mutagenesis
Study. J. Biol. Chem. 279, 42619-42627.
-
Alberola, T.M., García-Martínez, J., Antúnez,
O., Viladevall, L., Barceló, A., Ariño, J. &
Pérez-Ortín, J.E. (2004) A new set of DNA macrochips
for the yeast Saccharomyces cerevisiae: features and uses. Intl.
Microbiol. 7, 199-206.
-
Bernal, D., Serrano, R., Simón, E. & Ariño,
J. (2004) Copper and iron are the limiting factors for growth
of the yeast S. cerevisiae in an alkaline environment. J. Biol.
Chem. 279: 19698 - 19704.
-
Ruiz, A., Muñoz, I., Serrano, R., González, A.,
Simón, E. & Ariño, J. (2004) Functional characterization
of the Saccharomyces cerevisiae VHS3 gene. A regulatory subunit
of the Ppz1 protein phosphatase with novel, phosphatase-unrelated
functions. J. Biol. Chem. 279, 34421-34430.
-
Viladevall, L., Serrano, R., Ruiz, A., Doménech, G., Giraldo,
J., Barceló, A., & Ariño, J. (2004) Characterization
of the calcium-mediated response to alkaline stress in Saccharomyces
cerevisiae. J. Biol. Chem. 279, 43614-43624.
Reviews
& Book Chapters
-
Ariño, J.; Posas, F & Clotet, J. (1998) Isolation,
characterization and function of novel Ser/Thr protein phosphatases
in yeast. In: Methods in Molecular Biology: Protein Phosphatase
Protocols. Vol 93, pp. 305 313. (J.W. Ludlow, Ed.) Humana Press,
NJ.
- Pujol, G.; Ferrer, A. & Ariño, J.(1998) Protein Phosphatases
2A and protein Phosphatase X genes in Arabidopsis thaliana. In:
Methods in Molecular Biology: Protein Phosphatase Protocols. Vol
93, pp. 201-212. (J.W. Ludlow, Ed.) Humana Press, NJ.
-
Clotet, J. & Ariño, J. (2000) Nuevos componentes de
la regulación del ciclo celular. Investigación y
Ciencia. Octubre. Pp. 36-37.
-
Ariño, J. (2002) Novel protein phosphatases in the yeast:
an update. Eur. J. Biochem. 269, 1072-1077.
-
Ariño, J. & Herrero, E. (2003) Use of tetracycline-regulatable
promoters for functional analysis of protein phosphatases in yeast.
Methods in Enzymology. 366, 347-358.
-
Protein Phosphatases. (2004) In: Current Topics in Genetics. (J.
Ariño and D. Alexander Eds.) Springer-Verlag, Berlin.
-
Ariño, J., Casamayor, A., Ruiz, A., Muñoz, I., Marquina,
M. (2007) The use of yeast genetic tools to define biological
roles of novel protein phosphatases. In: Protein Phosphatase Protocols.
Methods in Molecular Biology, vol. 365, pp. 299-308. (G. Moorhead,
Ed.) Humana Press, NJ. (1-58829-711-X )
-
Ruiz, A. & Ariño, J. (2007) Function and Regulation
of the Saccharomyces cerevisiae ENA Sodium ATPase System. Eukaryotic
Cell 6, 2175-83.
Other
relevant information
-
Head of the DNA Microarray and Sequencing Facility at the Autonomous
University of Barcelona.
- Principal Investigator of a Research Group defined since 1994
as "High Quality Research Group" by the CIRIT (Generalitat
de Catalunya).
- Coordinator of the CIRIT Network "Molecular Biology of
Yeast".
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